Products & Services
NovoLux develops, produces and sells high-brightness fluorophores to increase the capabilities of existing multicolor flow cytometry equipment and testing methods. With our fluorophores, flow cytometer can be used to its full potential without expensive hardware and software upgrades. Furthermore, NovoLux's fluorophores have tunable brightness (a unique feature), meaning a single color can be made brighter to detect low abundance cells while not interfering with other colors.
NovoLux is targeting the most immediate needs for far-red and green colors. The high-brightness far-red fluorophores can overcome the low sensitivity of current signal detectors while the green fluorophores can eliminate false positive results due to low signal to noise detection (S/N). Over the next few years, NovoLux plans to develop products (additional colors) to cover the complete flow cytometry spectrum.
Our products can be immediately adopted. Unlike other fluorophore manufacturers that develop specific fluorophores individually, our platform technology can be quickly utilized across ALL colors and offers a drop-in solution compatible with current equipment and reagent analytic plate designs. Furthermore, our product increases the brightness of fluorophores across the entire visible range with minimal or no noise distortion.
Our Value Propositions:
- We provide researchers with high-brightness dyes to allow detection of previously undetectable cells and proteins.
- Our dyes allow reagent producers to develop new premium reagents.
- Flow cytometry manufacturers will be able to expand use of existing instruments capable of more than 28 colors.
Product, Technology & Competition
NovoLux provides high brightness fluorophores to create reagents for flow cytometry that are more than 10X brighter than any dyes currently in the industry. This brightness, combined with much sharper peaks allows the discovery of conditions that were not previously detectable.
Lab testing shows NovoLux outperforms products in all categories which include relative brightness, tunable brightness, photo stability, ease of conjugation (meets highest standard) and non-specific binding. The NovoLux reagents provide better resolution and accuracy while also adding the capability of using more than 6 colors during one test.
Our features include:
- More than 10x increase in brightness and sharp peaks, solving spillover and auto fluorescence issues.
- Brightness is tunable for specific cells – unique market feature
- Drop-in technology compatible with existing instrumentation
- Excellent water solubility and stability
- Easy to conjugate with antibodies
- Photostable, future growth to ELISA and microscopy
Flow cytometry is one of the most common clinical and lab testing methods used to detect diseased cells in blood. This highly efficient technique can examine thousands of blood cells per second, with potential to identify more than 28 types of cell structures simultaneously. This also allows a multi-parametric study of low abundance cells for early cancer detection and stem cell research. This simultaneous multi-parametric analysis technique offers a high throughput capability, and is routinely used in immunophenotyping, stem cell research, cell sorting, cell cycle analysis, apoptosis, and diagnosis of health disorders, such as blood cancers and HIV.
In practice, targeted cells in a sample are specifically stained with known fluorescence particles (fluorophores or dyes) in such a way that each specific cell types are stained with reagents that have known fluorescence colors. This fluidic sample is then passed through a flow cytometer, where the stained cells are shined by a laser for a few seconds. The light scattered in the forward direction gives information about the size of the cell, while light scattered from the side gives information about the cell structures. The laser induced fluorescence signal from the stained cells will be detected and counted by photo-multiplier tubes (PMTs) which correlate to their respective population. Further, separating these results by color allows the quantification of many different diseased cells present simultaneously. Such a high-throughput detection has made flow cytometry indispensable for clinical and research tests.
Currently, the equipment capability is constrained by the brightness of the fluorescence signals. Although the equipment is physically designed to run 28 colors, only a few are practically used. This issue can be partially overcome by expensive laser, laser filters, and by photo-multiplier tubes (PMTs). This creates problems for two reasons: 1) Spectral overlap (otherwise referred to as “spillover” in the industry) of typical dyes and 2) “auto-fluorescence” further interfering with accurate sample analysis, where inherent background fluorescence produced by various biostructures will be detected in addition to that given by the stain.
New Value to the Customer
With brighter fluorophores across the channel, NovoLux dyes minimize spillover due to the increase in the S/N ratio and substantially reduce the need for peak deconvolution data correction methods. Our tunable brightness also allows antigens on the cell surface to be expressed in different levels (some expressed at low level, some expressed at high level). The higher brightness and tunable nature of our fluorophores potentially enable more colors be utilized within a single sample test. This allows certain diseased cells to be identified that were previously shrouded by peak overlap and also solves the autofluorescence issue by giving signals that far outweigh background signals. Additionally, NovoLux’s tunable brightness also allows the precise increase in brightness of a single color to be chosen while not interfering with other signals. Overall, our product increases the S/N ratio across the entire spectra range.
All of this can be done without the need for equipment modifications or any deconvolution software and techniques. This makes NovoLux’s reagents a drop-in solution for seamless integration with current flow cytometry methods. Unlike other reagent suppliers that optimize one specific fluorophore one at a time, our platform technology offers a solution across the entire flow cytometry spectrum. Core biomed researchers can conduct faster testing with greater accuracy, meaning difficult research such as for stem cells, cancer, and multiple sclerosis, can be accelerated, leading to better treatment and improved patient outcomes. Additionally, rare events can be discovered, meaning NovoLux allows researchers to detect what was previously undetectable.
The major advantages of our products are the combined features of brightness, photostability, tunability and the absence of non-specific binding. Our products are also easy to conjugate with antibodies, which is important for strategic partners and end users. Furthermore, our dyes are at least 10X brighter than any other fluorophore available in the market.